Regulatory

Part:BBa_J119134

Designed by: MWSU Genetics Lab Fall 2012 2:00   Group: Eckdahl Lab   (2013-01-07)


pTac with mutations

This part was designed to see the effects of mutations on a functional promoter. Golden Gate Assembly was used to insert the mutations into Part J100091 at the double terminator sites. Mutation of pTac Promoter substitution of 2nd nt from an A to a T in the -35 region

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None